Download Analytical Ultracentrifugation VI by W. Mächtle, M. D. Lechner (auth.), W. Borchard, A. PDF

By W. Mächtle, M. D. Lechner (auth.), W. Borchard, A. Straatmann (eds.)

This quantity comprises 20 contributions of the twelfth assembly on Analytical Ultracentrifugation from March 1-2, 2001 in Duisburg, Germany. a variety of fields of ultracentrifugation are lined touching on learn difficulties in biochemistry, biophysical chemistry and macromolecular chemistry in addition to interacting structures. New investigations in regards to the sedimentation thought are awarded. The part transition of gels is handled, as is the sedimentation-diffusion equilibrium of gels. One part includes the hydrodynamics of biopolymers.

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3, where the best fit to the data yielded, besides (60 ± 10)% monomer, (40 ± 10)% dimer (averaged over the sample volume). The exact percentage and nature of the oligomer was barely dependent on the solvent but mainly, for unknown reasons, on the batch of the PEG derivative used. A higher average state of Fig. 3A, B Sedimentation equilibrium analysis of the PEG derivative based on v ¼ 0:903 ml=g and a monomer molar mass of 3,650 g/mol. A Experimental A(r) data collected at 345 nm (circles), fitted curve assuming the presence of monomers and dimers of the compound (—), and calculated local contributions of monomers (- - -) and dimers (ÆÆÆÆÆÆ).

As another consequence of the large size of the particles, light scattering strongly dominated the apparent light absorption of the sample. Detection of the boundary movement therefore used sample turbidity at 420 nm (a wavelength of relatively high lamp intensity). The large size of the HSA nanoparticles also led to virtually complete dominance of sedimentation with negligible diffusion. In combination with the high specific turbidity of the particles, which enabled the use of very low particle concentrations, this allowed treatment of their sedimentation as ideal and nondiffusing.

Durchschlag H, Zipper P (1997) J Appl Crystallogr 30:803 29. Merck & Co (1983) The Merck index, 10th edn. Merck & Co, Rayway 30. Weast RC, Selby SM (1967) Handbook of chemistry and physics, 48th edn. CRC, Cleveland 31. Ma¨chtle W (1992) In: Harding SE, Rowe AJ, Horton JC (eds) Analytical ultracentrifugation in biochemistry and polymer science. Royal Society of Chemistry, Cambridge, pp 147–175 32. Hermans JJ, Ende HA (1963) J Polym Sci Part C Polym Symp 1:161 33. Ma¨chtle W, Lechner MD (2001) Prog Colloid Polym Sci 34.

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